CAR-T construct design — guided through import, composer, plasmid and 3D view.

Name the functional blocks of a CAR · Explain why a CAR binds independently of MHC

Tell sequence formats apart and read a domain annotation · State a negative finding correctly

Assemble a construct from the library · Read, understand and fix a rule violation · Check a library entry against UniProt

Explain why a construct must fit into a vector

Describe why the distance to the target cell matters functionally

Name the approved indication areas · Recognise CRS and ICANS and grade them per ASTCT · Explain toxicity as a consequence of the mechanism

Build a 2nd-generation CD19 CAR following the architecture of tisagenlecleucel (Kymriah)